Codons code for specific amino acids in the genetic code. There are 20 standard amino acids that are coded for by the codons in the genetic code. Each amino acid is represented by one or more codons, with some amino acids having multiple codons that code for them. The genetic code is degenerate, meaning that multiple codons can code for the same amino acid. **

Start and stop codons are crucial in mRNA sequences because they signal the beginning and end of protein synthesis. The start codon, usually AUG, marks the point at which translation begins, and the ribosome starts assembling the amino acids into a protein. The stop codon, such as UAA, UAG, or UGA, signals the end of translation and the release of the completed protein. Without these codons, the ribosome would not know where to start or stop protein synthesis, leading to incomplete or nonfunctional proteins. Therefore, the presence and correct placement of start and stop codons are essential for proper protein production. **

There are 61 different tRNAs in biology, one for each codon. Each tRNA molecule carries a specific amino acid and has an anticodon sequence that is complementary to a specific codon in mRNA. This allows the tRNA to recognize and bind to the corresponding codon during protein synthesis. Therefore, with 61 codons, there are 61 different tRNAs in biology. **

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The Khorona method, developed by Marshall Nirenberg and Heinrich Matthaei in 1961, was instrumental in deciphering the genetic code and identifying stop codons. Using this method, they were able to synthesize RNA molecules of known sequences and observe the effects of adding different combinations of nucleotides to cell-free protein-synthesizing systems. Through their experiments, they were able to identify three specific nucleotide sequences (UAA, UAG, and UGA) as stop codons, which signal the termination of protein synthesis. Therefore, the Khorona method was indeed successful in deciphering stop codons and played a crucial role in understanding the genetic code. **

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Yes, the stop codons could potentially be deciphered using the method of Khorana. Khorana's work focused on deciphering the genetic code and determining the relationship between specific nucleotide sequences and the corresponding amino acids they encode for. Since stop codons also have specific nucleotide sequences that signal the end of protein synthesis, it is possible that Khorana's method could be applied to deciphering these sequences as well. However, further research and experimentation would be needed to confirm this hypothesis. **